Identification and Analysis on the Rice Blast Resistance Gene Pita in Wild Rice from Yunnan， China

doi:10.3969/j.issn.1001-7216.2014.06.014

Chinese Journal of Rice Science ›› 2014, Vol. 28 ›› Issue (6): 675-680.DOI: 10.3969/j.issn.1001-7216.2014.06.014

• Research Papers • Previous Articles

Identification and Analysis on the Rice Blast Resistance Gene Pita in Wild Rice from Yunnan， China

ZHAO Guozhen¹, JIANG Chunmiao ² , LIU Jixin¹, CHEN Yumin ¹ , YU Tengqiong ², CHENG Zaiquan ^2,*

¹ Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, China; ²Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;

Received:2014-03-11 Revised:2014-05-07 Online:2014-11-10 Published:2014-11-10
Contact: CHENG Zaiquan2,*

云南野生稻抗稻瘟病Pita基因的检测及分析

赵国珍¹,蒋春苗²,刘吉新¹,陈于敏¹ ,余腾琼² ,程在全 ^2,*

¹云南省农业科学院粮食作物研究所，昆明 650205；²云南省农业科学院生物技术与种质资源研究所，昆明 650223；

通讯作者: 程在全2,*
基金资助:
云南省科技攻关计划资助项目(2012BB013)；NSFC计划资助项目(U1302265，3160067)；农业行业专项(201003021)；云南省应用基础研究重点项目(2012FA005)。

Abstract

Abstract: A total of 16 accessions of wild rice from different regions of Yunnan Province were used for PCR amplification with Pita gene specific primers. The amplified PCR products were cloned into Topo cloning vector, and sequenced. Among 16 accessions, the identical sequence fragment to the resitance Pita gene was amplified separately from 7 accessions. The Pita allele in one accession of Oryza rufipogon with purple stem from Jinghong contains an identical DNA sequence in the coding region to the original Pita resistance gene. It suggested that Yunnan might be one of the origins of the Pita gene.

Key words: wild rice, rice blast resistance gene Pita, cloning, sequencing analysis

摘要： 以16个不同来源的云南野生稻作为供试材料，用抗稻瘟病Pita基因特异引物(KG2/KG4)进行PCR扩增，并对PCR产物进行克隆、测序及分析。结果表明，16个野生稻中7个品种持有抗稻瘟病Pita基因，其中，来自云南景洪的紫秆普通野生稻中Pita基因的编码区与原始型的抗稻瘟病Pita基因的DNA序列完全相同。可见，云南可能是抗稻瘟病基因Pita的起源地之一。

关键词: 野生稻, 抗稻瘟病Pita基因, 克隆, 测序分析

CLC Number:

ZHAO Guozhen1, JIANG Chunmiao2, LIU Jixin1 , CHEN Yumin1, YU Tengqiong2, CHENG Zaiquan2,* . Identification and Analysis on the Rice Blast Resistance Gene Pita in Wild Rice from Yunnan， China[J]. Chinese Journal of Rice Science, 2014, 28(6): 675-680.

赵国珍1,蒋春苗2,刘吉新1 ,陈于敏1 ,余腾琼2 ,程在全2,*. 云南野生稻抗稻瘟病Pita基因的检测及分析[J]. 中国水稻科学, 2014, 28(6): 675-680.

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Identification and Analysis on the Rice Blast Resistance Gene Pita in Wild Rice from Yunnan， China

云南野生稻抗稻瘟病Pita基因的检测及分析

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